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Camilla Forsberg
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Where does blood come from?
The Forsberg lab focuses on stem cell fate decisions of the blood system. Hematopoietic stem cells are responsible for generating a life-long supply of mature blood cells. Each stem cell is capable of making all of the mature blood cell types with widely different functions: some blood cells specialize in carrying oxygen, others fight off infections, and still others prevent bleeding in the process of blood clotting. How does a stem cell decide which cell type to give rise to? Are these decisions made by the stem cell itself, by its descendant multipotent progenitors, or both? How are these decisions dysregulated to cause cancer and other disorders?
We tackle these questions from multiple angles - by in vivo and in vitro experimental approaches, by focusing on specific molecules as well as analyzing global changes. Ultimately, we want to understand the molecular determinants of hematopoietic stem cell fate decisions so that we can prevent and treat both genetic and acquired disorders of the hematopoietic system, including anemia, autoimmune disease, leukemias and lymphomas.
Links:
Stem Cell Basics from NIH: http://stemcells.nih.gov/info/basics
Hematopoietic Stem Cells: http://stemcells.nih.gov/info/scireport/chapter5.asp
Postdoctoral fellow positions:
http://www2.ucsc.edu/ahr/academic_employment/bulletin_flyers_originaldocs_pdfs/07-08/T08-30.pdf
UCSC stem cell training program:
http://www.cbse.ucsc.edu/academics/cirm_scholars.shtml
UCSC Biomolecular Engineering:
http://www.bme.ucsc.edu/
Publications:
Santaguida, M., Schepers, K., King, B., Sabnis, A.J., Forsberg, E.C., Attema, J.L., Braun, B.S., and Passegué, E. (2009). JunB protects against myeloid malignancies by limiting hematopoietic stem cell proliferation and differentiation without affecting self-renewal. Cancer Cell, 5: 341-52.
Forsberg, E.C. and Smith-Berdan, S. (2009) Parsing the niche code: the molecular mechanisms governing hematopoietic stem cell adhesion and differentiation. Haematologica. In press.
Forsberg, E.C., Passegue, E1., Prohaska, S.S1., Wagers, A.J1., Koeva, M., Stuart, J.M., and Weissman, I.L. (2009) Molecular signatures of quiescent and mobilized hematopoietic stem cells and leukemic stem cells. In press.
Ooi, A.G.L., Karsunky, H., Majeti, R., Butz, S., Vestweber, D., Ishida, T., Quertermous, T., Weissman, I.L., and Forsberg, E.C. (2008) The adhesion molecule ESAM1 is a selective marker for hematopoietic stem cells. Stem Cells. 2008 Dec 11, Epub ahead of print.
Attema JL, Papathanasiou P, Forsberg EC, Xu J, Smale ST, Weissman IL. Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis. Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12371-6. Epub 2007 Jul 18.
Forsberg EC, Bhattacharya D, Weissman IL. Hematopoietic stem cells: expression profiling and beyond.
Stem Cell Rev. 2006;2(1):23-30. Review.
Forsberg EC, Serwold T, Kogan S, Weissman IL, Passegué E. New evidence supporting megakaryocyte-erythrocyte potential of flk2/flt3+ multipotent hematopoietic progenitors. Cell. 2006 Jul 28;
126(
2):
415-26.
Forsberg EC, Prohaska SS, Katzman S, Heffner GC, Stuart JM, Weissman IL. Differential expression of novel potential regulators in hematopoietic stem cells.
PLoS Genet. 2005 Sep;1(3):e28.
Forsberg EC, Bresnick EH. Histone acetylation beyond promoters: long-range acetylation patterns in the chromatin world. Bioessays. 2001 Sep;23(9):820-30. Review.
Forsberg EC, Downs KM, Christensen HM, Im H, Nuzzi PA, Bresnick EH.
Developmentally dynamic histone acetylation pattern of a tissue-specific chromatin domain.
Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14494-9.
Forsberg EC, Downs KM, Bresnick EH. Direct interaction of NF-E2 with hypersensitive site 2 of the beta-globin locus control region in living cells.Blood. 2000 Jul 1;96(1):334-9.
Forsberg EC, Johnson K, Zaboikina TN, Mosser EA, Bresnick EH. Requirement of an E1A-sensitive coactivator for long-range transactivation by the beta-globin locus control region.
J Biol Chem. 1999 Sep 17;274(38):26850-9.
Forsberg EC, Zaboikina TN, Versaw WK, Ahn NG, Bresnick EH. Enhancement of beta-globin locus control region-mediated transactivation by mitogen-activated protein kinases through stochastic and graded mechanisms. Mol Cell Biol. 1999 Aug;19(8):5565-75.
Mosser EA, Kasanov JD, Forsberg EC, Kay BK, Ney PA, Bresnick EH. Physical and functional interactions between the transactivation domain of the hematopoietic transcription factor NF-E2 and WW domains.
Biochemistry. 1998 Sep 29;37(39):13686-95.
Forsberg EC, Lam LT, Yang XJ, Nakatani Y, Bresnick EH.
Human histone acetyltransferase GCN5 exists in a stable macromolecular complex lacking the adapter ADA2. Biochemistry. 1997 Dec 16;
36(
50):
15918-24.
